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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2010 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. |
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Afiliação: |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
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Título: |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. |
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DOI: |
10.1590/S1516-635X2010000200005 |
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Idioma: |
Inglês |
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Conteúdo: |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). MenosAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais bef... Mostrar Tudo |
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Thesagro: |
Genética; Virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32305/1/Molecular-studies.pdf
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Marc: |
LEADER 02461naa a2200313 a 4500
001 1865545
005 2018-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1590/S1516-635X2010000200005$2DOI
100 1 $aABREU, J. T.
245 $aMolecular studies of the brazilian infectious bronchitis virus isolates.$h[electronic resource]
260 $c2010
520 $aAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
650 $aGenética
650 $aVirus
700 1 $aMOURÃO, M. M.
700 1 $aSANTOS, C. E.
700 1 $aVELOSO, C. J. M.
700 1 $aRESENDE, J. S.
700 1 $aFLATSCHART, R. B.
700 1 $aFOLGUERAS-FLATSCHAR, A. V.
700 1 $aJUNIOR, S. N.
700 1 $aSANTORO, M. M.
700 1 $aMENDES, A. C. R.
700 1 $aFRANCO, G. R.
700 1 $aSILVA, A.
700 1 $aCAMPOS, A. B.
700 1 $aFERNANDEZ, S.
773 $tRevista Brasileira de Ciência Avícola, Campinas$gv. 12, n. 2, p. 107-110, 2010.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
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Data corrente: |
13/08/2008 |
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Data da última atualização: |
30/01/2015 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
VERZIGNASSI, J. R.; RAMOS, A. K. B.; ANDRADE, C. M. S. de; FREITAS, E. M. de; LÉDO, F. J. da S.; GODOY, R.; ANDRADE, R. P. de; COELHO, S. P. |
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Afiliação: |
Jaqueline Rosemeire Verzignassi, Embrapa Gado de Corte; Allan Kardec Braga Ramos, Embrapa Cerrados; Carlos Maurício Soares de Andrade, Embrapa Acre; Evandro Macedo de Freitas, Unipasto; Francisco José da Silva Lêdo, Embrapa Gado de Leite; Ronaldo Pereira de Andrade, Embrapa Transferência de Tecnologia; Rodolfo Godoy, Embrapa Pecuária Sudeste; Sérgio Paulo Coelho, Superintendência Federal de Agricultura(SFA/MS). |
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Título: |
Tecnologia de sementes de forrageiras tropicais: demandas estratégicas de pesquisa. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Campo Grande, MS: Embrapa Gado de Corte, 2008. |
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Páginas: |
17 p. |
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Série: |
(Embrapa Gado de Corte. Documentos, 173). |
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Idioma: |
Português |
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Conteúdo: |
A produção de sementes de forrageiras tropicais representa um importante segmento da indústria de sementes no Brasil e há a necessidade do estabelecimento de prioridades para aumentar a eficiência do setor. Neste documento estão apresentados os resultados da discussão efetuada pelo grupo de trabalho reunido durante o Workshop "Tecnologia de Sementes de Forrageiras Tropicais: Demandas Estratégicas de Pesquisa", realizado pela Embrapa Gado de Corte em abril de 2008. As demandas de pesquisa discutidas e consideradas prioritárias foram pertinentes a vários aspectos da produção e tecnologia de sementes de forrageiras tropicais, da semeadura ao processamento, bem como ao armazenamento e análises laboratoriais. |
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Palavras-Chave: |
Brasil; Controle; Gramíneas; Leguminosas; Production; Tropical forages. |
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Thesagro: |
Adubação; Comercialização; Dormência; Forrageira Tropical; Germinação; Pastagem; Pesquisa; Produção; Semente; Tecnologia. |
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Thesaurus NAL: |
Brazil; commercialization; pastures; research; seeds; technology. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPGC/12055/1/Doc173.pdf
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Marc: |
LEADER 01994nam a2200481 a 4500
001 1326562
005 2015-01-30
008 2008 bl uuuu u0uu1 u #d
100 1 $aVERZIGNASSI, J. R.
245 $aTecnologia de sementes de forrageiras tropicais$bdemandas estratégicas de pesquisa.$h[electronic resource]
260 $aCampo Grande, MS: Embrapa Gado de Corte$c2008
300 $a17 p.
490 $a(Embrapa Gado de Corte. Documentos, 173).
520 $aA produção de sementes de forrageiras tropicais representa um importante segmento da indústria de sementes no Brasil e há a necessidade do estabelecimento de prioridades para aumentar a eficiência do setor. Neste documento estão apresentados os resultados da discussão efetuada pelo grupo de trabalho reunido durante o Workshop "Tecnologia de Sementes de Forrageiras Tropicais: Demandas Estratégicas de Pesquisa", realizado pela Embrapa Gado de Corte em abril de 2008. As demandas de pesquisa discutidas e consideradas prioritárias foram pertinentes a vários aspectos da produção e tecnologia de sementes de forrageiras tropicais, da semeadura ao processamento, bem como ao armazenamento e análises laboratoriais.
650 $aBrazil
650 $acommercialization
650 $apastures
650 $aresearch
650 $aseeds
650 $atechnology
650 $aAdubação
650 $aComercialização
650 $aDormência
650 $aForrageira Tropical
650 $aGerminação
650 $aPastagem
650 $aPesquisa
650 $aProdução
650 $aSemente
650 $aTecnologia
653 $aBrasil
653 $aControle
653 $aGramíneas
653 $aLeguminosas
653 $aProduction
653 $aTropical forages
700 1 $aRAMOS, A. K. B.
700 1 $aANDRADE, C. M. S. de
700 1 $aFREITAS, E. M. de
700 1 $aLÉDO, F. J. da S.
700 1 $aGODOY, R.
700 1 $aANDRADE, R. P. de
700 1 $aCOELHO, S. P.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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